Table Of Content

Journal of Immunological Methods, 151 (1992) 318-319 © 1992 Elsevier Science Publishers B.V. All rights reserved 0022-1759 /92 /$05.00 Contents to volume 151 (1992) Numbers 1-2 — 6 July 1992 Research reports Dextran, a hapten carrier in immunoassays for S-triazines. A comparison with ELISAs based on hapten-protein conjugates M. Bécher, T. Giersch and R.D. Schmid (Germany) An ELISA utilizing immobilised snowdrop lectin GNA for the detection of envelope glycoproteins of HIV and SIV N. Mahmood and A.J. Hay (UK) Production of epitope specific monoclonal IgG antibodies to HLA class II molecules by combining in vivo and in vitro immunization J.S. Petersen and T. Dyrberg (Denmark) Studies of the low dose ‘hook’ effect in a competitive homogeneous immunoassay S.A. Fernando, J.R. Sportsman and G.S. Wilson (USA) Studies of the ‘hook’ effect in the one-step sandwich immunoassay S.A. Fernando and G.S. Wilson (USA) Multiple epitope interactions in the two-step sandwich immunoassay S.A. Fernando and G.S. Wilson (USA) A simple approach to improving sensitivity in a one-step monoclonal antibody-based ELISA for human IIbIIla using multiple conjugates A.M. Rowland, M.A. Wooldridge, K.A. Rosenthal and W.L.T. Wong (USA) Evaluation of labelled monoclonal antibodies by simultaneous estimation of the association constant, the immunoreactive fraction, and the number of effective binding sites on the specific target J.G. Field and A. Skretting (Norway) Electroporation of CTL clones: a useful method to investigate signalling pathways leading to the expression of effector functions C. Langlet and A.-M. Schmitt-Verhulst (France) The natural anti-a-galactosyl IgG in seminal fluid. A simple means to determine damage to the blood-genital tract barrier in infertile males U. Galili, M. Ron and R. Sharon (Israel) A simple technique for the determination of x and A immunoglobulin light chain expression by B cells in whole blood W.M. Reynolds, A.M. Williamson, G.J. Smith and A.C. Lane (UK) Measurement of PAF in blood by radioimmunoassay. Examination of interfering factors and problems involved in accurate quantification S.J. Cooney, M.A. Smal and B.A. Baldo (Australia) Use of soluble recombinant TNF receptor to improve detection of TNF secretion in cultures of tumor infiltrating lymphocytes P. Hwu, S. Schwarz, M. Custer, C_A. Smith, J.J. Mulé and S.A. Rosenberg (USA) Monoclonal antibody mediated targeting of enzymes. A comparative study using the mouse spleen as a model system N. Van Rooijen, H. Ter Hart, G. Kraal, N. Kors and E. Claassen (Netherlands) Analyses of affinity distributions within polyclonal populations of antigen-specific antibodies. Evaluation of their accuracy in population detection using monoclonal antibodies U. Bruderer, M. Deusinger, U. Schiirch and A.B. Lang (Switzerland) Production and characterisation of monoclonal antibodies against native and disassembled human catalase E.A.C. Wiemer, R. Ofman, E. Middelkoop, M. De Boer, R.J.A. Wanders and J.M. Tager (Netherlands, Belgium) The development of a quantitative assay for the detection of anti-Ro/SS-A and anti-LA/SS-B autoantibodies using purified recombinant proteins C.H.A. Veldhoven, J.F. Meilof, J.G. Huisman and R.J.T. Smeenk (Netherlands) In situ immunocytochemical detection of cells containing antibodies specific for Eimeria tenella antigens L. Vervelde, A.N. Vermeulen and S.H.M. Jeurissen (Netherlands) Production of a functional monoclonal antibody recognizing human colorectal carcinoma cells from a baculovirus expression system M. Nesbit, Z.F. Fu, J. McDonald-Smith, Z. Steplewski and P.J. Curtis (USA) Journal of Immunological Methods, 151 (1992) 318-319 © 1992 Elsevier Science Publishers B.V. All rights reserved 0022-1759 /92 /$05.00 Contents to volume 151 (1992) Numbers 1-2 — 6 July 1992 Research reports Dextran, a hapten carrier in immunoassays for S-triazines. A comparison with ELISAs based on hapten-protein conjugates M. Bécher, T. Giersch and R.D. Schmid (Germany) An ELISA utilizing immobilised snowdrop lectin GNA for the detection of envelope glycoproteins of HIV and SIV N. Mahmood and A.J. Hay (UK) Production of epitope specific monoclonal IgG antibodies to HLA class II molecules by combining in vivo and in vitro immunization J.S. Petersen and T. Dyrberg (Denmark) Studies of the low dose ‘hook’ effect in a competitive homogeneous immunoassay S.A. Fernando, J.R. Sportsman and G.S. Wilson (USA) Studies of the ‘hook’ effect in the one-step sandwich immunoassay S.A. Fernando and G.S. Wilson (USA) Multiple epitope interactions in the two-step sandwich immunoassay S.A. Fernando and G.S. Wilson (USA) A simple approach to improving sensitivity in a one-step monoclonal antibody-based ELISA for human IIbIIla using multiple conjugates A.M. Rowland, M.A. Wooldridge, K.A. Rosenthal and W.L.T. Wong (USA) Evaluation of labelled monoclonal antibodies by simultaneous estimation of the association constant, the immunoreactive fraction, and the number of effective binding sites on the specific target J.G. Field and A. Skretting (Norway) Electroporation of CTL clones: a useful method to investigate signalling pathways leading to the expression of effector functions C. Langlet and A.-M. Schmitt-Verhulst (France) The natural anti-a-galactosyl IgG in seminal fluid. A simple means to determine damage to the blood-genital tract barrier in infertile males U. Galili, M. Ron and R. Sharon (Israel) A simple technique for the determination of x and A immunoglobulin light chain expression by B cells in whole blood W.M. Reynolds, A.M. Williamson, G.J. Smith and A.C. Lane (UK) Measurement of PAF in blood by radioimmunoassay. Examination of interfering factors and problems involved in accurate quantification S.J. Cooney, M.A. Smal and B.A. Baldo (Australia) Use of soluble recombinant TNF receptor to improve detection of TNF secretion in cultures of tumor infiltrating lymphocytes P. Hwu, S. Schwarz, M. Custer, C_A. Smith, J.J. Mulé and S.A. Rosenberg (USA) Monoclonal antibody mediated targeting of enzymes. A comparative study using the mouse spleen as a model system N. Van Rooijen, H. Ter Hart, G. Kraal, N. Kors and E. Claassen (Netherlands) Analyses of affinity distributions within polyclonal populations of antigen-specific antibodies. Evaluation of their accuracy in population detection using monoclonal antibodies U. Bruderer, M. Deusinger, U. Schiirch and A.B. Lang (Switzerland) Production and characterisation of monoclonal antibodies against native and disassembled human catalase E.A.C. Wiemer, R. Ofman, E. Middelkoop, M. De Boer, R.J.A. Wanders and J.M. Tager (Netherlands, Belgium) The development of a quantitative assay for the detection of anti-Ro/SS-A and anti-LA/SS-B autoantibodies using purified recombinant proteins C.H.A. Veldhoven, J.F. Meilof, J.G. Huisman and R.J.T. Smeenk (Netherlands) In situ immunocytochemical detection of cells containing antibodies specific for Eimeria tenella antigens L. Vervelde, A.N. Vermeulen and S.H.M. Jeurissen (Netherlands) Production of a functional monoclonal antibody recognizing human colorectal carcinoma cells from a baculovirus expression system M. Nesbit, Z.F. Fu, J. McDonald-Smith, Z. Steplewski and P.J. Curtis (USA) Solid phase enzyme immunoassay of cyclic adenosine 3’,5’-monophosphate. Effect of coating strategy upon assay perfor- mance in comparison with radioimmunoassay J. Linden, G.E. Vandenhoff, D. Taylor and G.L. Finkelstein (USA) The effect of the temperature and duration of sample storage on the measurement of lymphocyte subpopulations from HIV-1-positive and control subjects T. Ekong, A.M. Hill, M. Gompels, A. Brown and A.J. Pinching (UK) A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific y isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences M.E. Duncan, S.M. McAleese, N.A. Booth, W.T. Melvin and J.E. Fothergill (UK) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53 B. VojtéSek, J. Bartek, C.A. Midgley and D.P. Lane (UK, Czechoslovakia) Fluorometric determination of the DNA content of cells cultured in tissue culture plates M. Goppelt-Struebe and M. Golombek (Germany) Avidin-binding to peripheral blood B lymphocytes and monocytes C. Andreoni and M. Delaage (France) A modified colorimetric method for the measurement of phagocytosis and antibody-dependent cell cytotoxicity using 2,7-diaminofluorene S.J. Gebran, E.L. Romano, H.A. Pons, L. Cariani and A.N. Soyano (Venezuela) A novel B cell stimulation/proliferation assay using simultaneous flow cytometric detection of cell surface markers and DNA content J. Storek, I. Schmid, S. Ferrara and A. Saxon (USA) Improved enzyme immunosorbent assay for mouse prolactin using penicillinase as label Y. Tomita, R.W. Engelman, I. Bauer-Sardina, N.K. Day and R.A. Good (USA) A polymerase chain reaction assay for the detection and quantitation of cytokine gene expression in small numbers of cells S.R. Carding, D. Lu and K. Bottomly (USA) A blocking ELISA for the detection of specific antibodies to bovine ephemeral fever virus H. Zakrzewski, D.H. Cybinski and P.J. Walker (Australia) A method for the detection of serologically crossreacting antigens both within and between protein mixtures: the Western cross blot P. Hammerl, A. Hartl, J. Freund and J. Thalhamer (Austria) Short communication A simple method of concentrating monoclonal antibodies from culture supernatant by ultrafiltration K. Saha, R. Case and P.K.Y. Wong (USA) Letters to the editors Factors influencing computer-assisted video image analysis of immunocytochemically stained lymphocytes and macrophages in the spleen of mice W. Baumgartner, E. Atzpodien, H. Weintraut and G. Seibold (Germany) The L929 cell bioassay for murine tumour necrosis factor is not influenced by other murine cytokines P. Tomkins, K. Cooper, D. Webber and G. Bowen (UK) Announcement Contents to volume 151 (1992) Notes to authors