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इंटरनेट मानक Disclosure to Promote the Right To Information Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public. “जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ” Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru “The Right to Information, The Right to Live” “Step Out From the Old to the New” IS 15344 (2003): Green Tea [FAD 6: Stimulant Foods] “!ान $ एक न’ भारत का +नम-ण” Satyanarayan Gangaram Pitroda ““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee”” “!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै” Bhartṛhari—Nītiśatakam “Knowledge is such a treasure which cannot be stolen” IS 15344:2003 ( Wwfhmw sit m – milk Indian Standard GREEN TEA — SPECIFICATION ICS 67.140.10 0 BIS 2003 BUREAU OF INDIAN STANDARDS MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG NEW DELHI 110002 .4u/yNt2003 Price Group 3 AMENDMENT NO. 1 JUNE 2011 TO IS 15344 : 2003 GREEN TEA — SPECIFICATION [Page 2, clause 7.1.1(c)] — Substitute ‘Net quantity’ for ‘Net mass’. [Page 2, clause 7.1.2(c)] — Substitute ‘Net quantity’ for ‘Net mass’. [Page 2, clause 7.1.2(e)] — Substitute ‘Tare quantity’ for ‘Tare mass’. (FAD 6) Reprography Unit, BIS, New Delhi, India Stimulant Foods Sectional Committee, FAD 23 FOREWORD This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Stimulant Foods Sectional Committee had been approved by the Food and Agriculture Division Council. Tea isapopular beverage consumed all over the world. It isan important commodity in the international trade and India isone of the major tea producing and exporting countries inthe world. Earlierboth BlackTeaandGreenTeawerecovered inIS3633: 1972.Onrevision ofIS3633technical committee felttheneedtobring out aseparate standard forgreen tea,inlinewiththepractice ofISO, which isintheprocess of developing aseparate standard for Green Tea. Theobjects ofthisstandard aretospecify theplant source fromwhich Green Teaistobemanufactured andtoset requirements for certain chemical characteristics which, if met, are an indication that the Green Tea has been subjected to recognized good production practice. To save time and expenses, the quality of Green Tea isalso assessed by tea taster, who from his experience can assess whether a given Green Tea would comply with the chemical requirements or not. Inthepreparation ofthis standard, dueconsideration hasbeen, given tothePrevention ofFoodAdulteration Act, 19s4 and the Rules framed thereunder and the Standards of Weights and Measures (Packaged Commodities) Rules, 1977. The standard is,however, subject tothe restrictions imposed under these, wherever applicable. For the pu~ose of deciding whether aparticular requirement of this standard iscomplied with, the final value, observed or calculated, expressing the result of a test or analysis, shall be rounded off in accordance with IS2: 1960‘Rules forrounding offnumerical values (revised)’. The number ofsignificant places retained inthe rounded off value should be the same asthat ofthe specified value inthis standard. IS 15344:2003 Indian Standard GREEN TEA — SPECIFICATION 1SCOPE ISNo. Title” 14437:1997 Method for determination of This standard prescribes therequirements, methods of quinalphos residues in agricultural test and sampling for Green Tea. and food commodities 2REFERENCES 3TERMINOLOGY The following standards contain provisions; which GreenTeameanstheteaderived solelyandexclusively through reference in this text, constitute provision of andproduced byacceptable processes, notably enzyme this standard. At the time of publication, the editions inactivation, rolling orcomminution and drying, from indicated were valid. All standards are subject to the leaves, buds and tender stems of varieties of the revision and parties to agreements based on this species Camellia sinensis (Linnaeus) O. Kuntze, standard are encouraged to investigate the possibility known to be suitable for making Green Tea for of applying the most recent editions of the standards consumption asabeverage. indicated below: 4REQUIREMENTS [SNo. Title 1070:1992 Reagent gradewater(third revision) 4.1 Description 2491:1998 Food hygiene — General principles TheGreen Tea shallproduce aliquor ofcharacteristic —Codeofpractice(second revision) flavour, colour andtaste. The Green Tea shallhave no 3611:1975 Method of sampling for tea @rst taint and shall be free from extraneous matter, added revision) colours and non-permitted flavours. Green Tea shall 3633 ;2003 Black Tea (second revision) be evaluated in accordance with the procedure 10226 Method for determination of crude prescribed inAnnex A of IS 3633. (Part 1): 1982 tibre content: Part 1General method 11123:1984 Method for determination ofcopper 4.2Ironfilings intheproduct shallnotexceed thelimit by atomic absorption spectro- of 250 mg/kg, and their size shall not be greater than photometry 2.0 mm when tested by the method given inAnnex C 11773:1986 Method for determination of ethion of IS 3633. residues in food commodities, soil NOTE — No limit is specified for the moisture content of the and water Green Tea. Ifdesired, the actual loss in mass at 103+ 2°C of 12074:1987 Method fordetermination ofleadby thesample asreceived maybe determined andtheresult recorded atomic absorpti on spectro- inthetest report. Insuch cases, thedetermination shall becarried out by the method described in1S13853. photometry 13852:1994 Tea—Preparation ofgroundsample 4.3 Hygiene Requirements of known dry matter content Green Tea shall be manufactured and packed under 13853:1994 Tea—Determination oflossinmass hygienic conditions asper 1S2491, at 103”C 13854:1994 Tea— Determination oftotal ash 4.4 Chemical Requirements 13855:1993 Tea — Determination of water- 4.4.1 Green Tea shall also comply with the soluble ashand water-insoluble ash requirements specified in Tables 1,2 and 3 in which 13856:1993 Tea — Determination of alkalinity allthe requirements are expressed on the basis of the ofwater-soluble ash material oven-dried at 103 + 2°C by the method 13857:1993 Tea — Determination of acid- described inIS 13852. insoluble ash 13859:1993 Instant tea in solid form — 5 ADDITIONAL REQUIREMENT FOR ECO- Determination of moisture content MARK (loss inmass at 103°C) 13862:1998 Tea — Determination of water The additional requirement for ECO-Mark are same extract (@t revision) asgiven inAnnex Bof IS 3633. 14629:1999 Method fordetermination ofdicofol 6PACKING residues in agricultural and food commodities Green Tea shall be packed in closed, clean and dry 1 IS 15344:2003 containers made ofmaterial, which doesnot affect the b) Name of the product; Green Tea, or inaccordance with the customary trade c) Net mass of contents; practices so as to allow the Green Tea to retain its d) Month and year of manufacture; freshness. e} Batch, code number or any other Table 1Requirements for Green Tea identification No.; (Clause 4.4.1) f) Thewords ‘Bestbefore........’(Monthandyear tobe indicated); SI Characteristic Requirement Method of g) Tea board registration number, if flavour is No. Test, Ref to added; 1SNo. Annex of” h) Declaration, if flavours and flavoring this standard substance added; and (1) (2) (3) (4) j) Any other requirements given under the i} Waterextract, 32.0 13862 — Prevention ofFood Adulteration Rules, orthe percent by mass, Standards of Weights and Measures Mi/f (Packaged Commodities) Rules, 1977. ii) Total ash, percent 4.0-8.0 13854 — by mass, 7.L2 Wholesale Package iii) Water-soluble ash, 45 13855 oftotal ash, percent a) Name and address of the manufacturer; by mass, &fin b) Name of the product; iv) Alkalinity of water- 1.0-2.2 13856 – soluble ash c) Netmass of contents; (as KzO), percent by d) Gross mass of contents; mass v) Acid-insoluble ash, 1.0 13857 — e) Tare mass of contents; percent by mass, f) Month and year of manufacture; Max vi) Crude tibre, percent 16.5 10226 g) Batch, code number or any other by mass, Max (Part 1) identification No.; vii) Tordl catechins, 9.0 Annex A h) The words ‘Best before .........’ (Month and percent by mass, year tobe indicated); Min j) Tea board registration number, if flavour is Table 2Additional Requirements — added; Metallic Contaminants k) Declaration, if flavours and flavoring substance added; and (Clause 4.4.1) m) Any other requirements given under the SI Characteristic Requirem e nt Method of Prevention ofFood Adulteration Rules, orthe No. Test, Ref to Standards of Weights and Measures (1) (2) (3) (4) (Packaged Commodities) Rules, 1977. i) Lead, mg/kg, Max 10.0 IS 12074 7.2 BIS Certification Marking ii) Cormer. me/ke. Max 150.0 IS 11123 The product may also be marked with the Standard Table 3 Additional Requirements — Mark. Pesticide Residues 7.2.1 TheuseoftheStandard Mark isgoverned bythe (Clause 4.4.1) provisions of Bureau of Indian Standards Act, 1986 and the Rules and Regulations made thereunder. The SI Characteristic Requirement Method ofTest, details of conditions under which the licence for the No. Ref to use of the Standard Mark may be granted to (1) (2) (3) (4) manufacturers orproducers maybe obtained from the i) Dicofol, mg/kg, Max 5.0 IS 14629 Bureau of Indian Stand&ds. ii) Ethion, mg/kg, Max 5.0 IS 11.773 iii) Quinolphos, mg/kg, Max 0.01 Is 14437 8SAMPLING 7MARKING The ground sample of the material shall be prepared 7.1 The information given in 7.1.1 or 7.1.2 shall be inaccordance with theprocedure outlined inIS 13852 clearly and indelibly marked on the package. before undertaking the analysis for various chemical characteristics. The method ofdrawing representative 7.1.1 Retail Package samples ofthematerial andthe criteria forconformity a) Name and address of the manufacturer; shall be asprescribed inIS 3611. 2 IS 15344:2003 ANNEX A [Table 1 Item (vii)] DETERMINATION OF CATECHINS IN GREEN TEA — METHOD USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY A-1 PRINCIPLE sufficient quantities (that is >20 mg) an analytical balance capable of weighing to at least 0.1 mg is The total catechin content is extracted from a test required, whereas for limited quantities (that is <20 portion offinely ground teawith 70percent methanol mg) ananalytical balance capable ofweighing to0.01 at70”C,and instant tea isdissolved inhot water with mg isrequired. 10 percent (v/v) acetonitrile added to stabilize the extract. The resulting extracts are then assayed for Weighed amounts of standards are transferred to individual intact catechins by HPLC on a phenyl separate one-mark volumetric flasks and dissolved bonded column using a linear acetonitrile gradient in stabilizing solution, gently warming, if required elution and detection by UV absorbance at a (maximum 40°C). The cool solution isdiluted to the wavelength of278 nm. mark with stabilizing solution. Stock standard solutions are prepared in accordance with A-2.10.2 A-2 REAGENTS to A-2.1O.8. A-2.1 Water — inaccordance with IS 1070. A-2.10.2 Gallic Acid Stock Standard Solution — A-2.2 Acetonitrile — HPLC Grade. corresponding to 2.00 mg/ml. A-2.3 Methanol A-2.1O.3 Caffeine Stock Standard Solution — corresponding to2.00 mg/ml. A-2.4 Glacial Acetic Acid — HPLC Grade. A-2.1O.4(+)–Catechin, + C, Stock Standard Solution A-2.5 EDTA (Ethylenediaminetetraacetic Acid — corresponding to 1.00mg/ml. Disodium Salt, Dihydrate) A-2.1O.5(–)-Epicatechin, EC, Stock Standard Solution A-2.6 L-ascorbic Acid — Free acid. — corresponding to 1.00mg/ml, A-2.7 Methanol/Water Extraction Mixture, A-2.10.6 (–)–Epigallocatechin, EGC, Stock Standard 70 percent v/v Methanol — Add 700 ml of the Solution — corresponding to 2.00 mgfml. methanol to a 1litre mark volumetric flask. Dilute to the mark with water and mix. A-2.10.7 (–) –Epigallocatech ingallate, EGCG, Stock Standard Solution — corresponding to 2.00 mg/ml. A-2.8 HPLC Mobile Phase — Cautio n wear gloves, eye protection and dispense reagents in a fume A-2.1 0.8 (–) –EpicatechingaIla te, ECG, Stock cupboard. * Standard Solution — corresponding to 2.00 mglml. A-2.8.1 Mobile Phase A—Add 180mlofacetonitrile A-2.11 Dilute Gallic Acid Standard Solution — and40mlaceticacidtoa2litremarkvolumetric flask. corresponding to 200 pg/ml. Using a pipette transfer Dilute tothe mark with water, mix, and filter through 10ml of the gallic acid stock standard solution to a a filter of 0.45 pm pore size. 100mlone-mark volumetric flask. Dilute tothemark A-2.8.2Mobile Phase B—Add 800mlacetonitriletoa with stabilizing solution and mix. 1Iitre mark vohunetric flask. Dilute to the mark with A-2.12 Mixed Working Standard Solutions water, mix and filter through a filter of 0.45 pm pore size. A-2.12.1 Prepare three mixed working standard solutions, with concentrations selected to cover the A-2.9 Stabilizing Solution range of compositions typically found intea. Weigh,tothenearest0.01g,0.25gofEDTAand0.25g A-2.12.2Following Table4,carefullypipette thegiven of ascorbic acid into a 1litre mark volumetric flask aliquotsofdilutegallicacidstandald solutionandstock anddissolve inapproximately 500mlwater. Add 100 standard solutions into three separate 20 mlone-mark mlacetonitrile dilute tothe mark with water and mix. volumetric flasks, dilute to volume with stabilizing Prepare fresh stabilizing solution on the day ofuse. solution and mix. These mixed working standard A-2.1OStock Standard Solutions solutions correspond to the nominal concentrations showninTable5.Usetheactualstandardweightstaken A-2.1O.I General — When standards are available in 3 IS 15344:2003 Table 4 Composition of Mixed Working Standard Solutions Standard 1to Standard 3 (Clause A-2.12.2) SI Component Solution Aliquot, ml No. Standard 1 Standard 2 Standard 3 (1) (2) (3) (4) (5) (6) i) Gallic acid 200 pg /ml dilute stock standard solution 0.5 1.0 2.5 ii) Caffeine 2.00 mg/ml stock standard solution 0.5 1.0 1.5 iii) +C 1.00 mg/ml stock standard solution 1.0 2.0 3.0 Iv) EC 1.00 mg/ml stock standard solution 1.0 2.0 3.0 v) EGC 2.00 mg/ml stock standard solution 1.0 2.0 3.0 vi) EGCG 2.00 mg/ml stock standard solution 1.0 2.0 4.0- vii) ECG 2.00 mg/ml stock standard solution 0.5 1.0 2.0 to obtain the actual concentrations at each standard A-3.6Extraction Tubes — glass, 10 ml capacity, level. stoppered and able towithstand centrifugation. A-3.7 Graduated Tubes —glass, 10mlcapacity with Table 5Nominal Concentrations in 0.1 ml graduations. Mixed Working Standard Solutions Standard 1to Standard 3 A-3.8 Automatic Pipettes — to cover the volume range for standard and sample extract dilutions. (Clause A-2.12.2) A-3.9 Filters —membrane filterunitsof0.45 pmpore S1No. Component Nominal Concentration size. Standard 1 Standard 2 Standard 3 A-3.1OHigh-Performance Liquid Chromatography (1) (2) (3) (4) (5) —equipped toperform binary gradient elution, with a 1) Gallic acid 5 10 25 thermostatically controlled column compartment and ii) Caffeine 50 100 150 an ultraviolet detector, allowing measurements to be iii) +C 50 100 150 made atawavelength of 278 nm. l\J) Ec 50 100 150 v) EGC 100 200 300 A-3.11Data Collection/Integration System vi) EGCG 100 200 400 vii) ECG 50 100 200 A-3.12 Chromatographic Column for HPLC A-2.12.3Pipette 1.0mlaliquotsofeachmixedstandard NOTES solutionintolabelledsmaliamberg lassvials,gentlyflush 1Phenyl bonded phases give additional selectivity over reversed withnitrogenprior tosealingandstor efrozenat–20°C. phase packings, and result in improved resolution of the catechins. NOTES 2 In this standard the chromatographic conditions and 1 Mixed working standard solutions are stable for at least composition of the mobile phase specified are suitable for a 2months when stored frozen at–20”C. Phenomenex Lures 5Lm Phenyl-Hexyl column of dimensions 2 Only thaw sufficient mixed working standard solution vials 250 mm x 4.6 mm fitted with a Phenomenex Security Guard foreach chromatographic run. Discard any remaining solution, 4mm x3.0mmPhenyl-Hexyl cartridge. Ifother types ofcolumn do not re-freeze. are used, an alternative mobile phase and alternative chromatographic conditions may be necessary. A-3 APPARATUS A-4 SAMPLING A-3.1 Analytical Balance — capable of weighing to It is important that the laboratory receives a sample an accuracy of* 0.0001 g. whichistrulyrepresentative andhasnotbeendamaged A-3.2 Water Bath — regulated to a temperature of or changed during transport or storage. 70* 1“c. A-5 PREPARATION OF TEST SAMPLES A-3.3Dispenser — set at 5 ml for methanol/water extraction mixture (see A-2.7). Grind thesample ofGreen leaftea orBlack leafteain accordance with IS 13852. Store all samples in well A-3.4 Centrifuge — capable of 3500 rev/rein. sealed containers, protected from light, and cool. A-3.5 Vortex Mixer — for efficient mixing during NOTE — Grinding of instant tea is only required forsamples extraction. with acoarse granular structure. 4