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interactions of transmembrane peptides and proteins with lipid membranes PDF

137 Pages·2005·4.12 MB·English
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Preview interactions of transmembrane peptides and proteins with lipid membranes

Interactions of transmembrane peptides and proteins with lipid membranes studied by mass spectrometry Interactions of transmembrane peptides and proteins with lipid membranes studied by mass spectrometry Interacties van transmembraan peptiden en eiwitten met lipide membranen bestudeerd met behulp van massaspectrometrie (met een samenvatting in het Nederlands) Proefschrift ter verkrijging van de graad van doctor aan de Universiteit Utrecht op gezag van de Rector Magnificus, prof. dr. W. H. Gispen, ingevolge het besluit van het College voor Promoties in het openbaar te verdedigen op woensdag 27 november 2002 des middags te 12.45 uur door Jeroen Adrianus Antonius Demmers geboren op 7 september 1975, te Bergen op Zoom Promotoren: Prof. dr. J. A. Killian Prof. dr. A. J. R. Heck Prof. dr. J. Haverkamp verbonden aan de Faculteit Scheikunde der Universiteit Utrecht ISBN: 90-393-3170-7 Omslag: Fragment van ‘Buste d’homme’, door Pablo Picasso (1969) Druk: Ridderprint Offsetdrukkerij B.V., Ridderkerk Het in dit proefschrift beschreven onderzoek werd gefinancierd door het gebied Chemische Wetenschappen (CW) van de Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). “Human life occurs only once, and the reason we cannot determine which of our decisions are good and which are bad is that in a given situation we can make only one decision; we are not granted a second, third, or fourth life in which to compare various decisions.” (cid:1) Milan Kundera in The Unbearable Lightness of Being (1984) Aan mijn ouders Table of contents Chapter 1 General introduction 9 Chapter 2 Electrospray ionization mass spectrometry as a novel tool to analyze hydrogen/deuterium exchange kinetics of transmembrane peptides in lipid bilayers 41 Chapter 3 Interfacial positioning and stability of transmembrane peptides in lipid bilayers studied by combining hydrogen/deuterium exchange and mass spectrometry 57 Chapter 4 Factors affecting gas-phase deuterium scrambling in peptide ions and their implications for protein structure determination 77 Chapter 5 Interaction of the K+ channel KcsA with membrane phospholipids studied by electrospray ionization mass spectrometry 97 Chapter 6 Summarizing discussion 109 List of abbreviations 125 Nederlandse samenvatting 127 Curriculum vitae 132 List of publications 133 Dankwoord 134 CHAPTER 1 General introduction Chapter 1 10

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