Hyperspectral Remote Sensing in Detection of Biotic and Abiotic Stresses in Plants for Precision Agriculture Keshav D Singh (Postdoc); Christian Nansen (P.I.); Robert Starnes (Pilot); Elvira De Lange (Postdoc); UC Davis, CA 1 Change in Spectral Signature Agriculture Plants/Trees Hyperspectral Imaging Pest Management Infestation/Disease/Wilt Pesticides Spray Biotic/Abiotic Stress Crops Management/ Vegetation starts Damaging Precision Agriculture 1 Hyperspectral Imaging (HSI): Imaging Spectroscopy: Interaction • Imaging and Spectroscopy between the electromagnetic • Hundreds of narrow spectral radiation and contact material bands to address subtle information Advantages: • Non-destructive • Low cost • Less time 2 Ref: @Basics of Crop Sensing, ANR-1398 Plant-Biotic Interaction: ! Infestation/Pests ! Fertilizers/Nutrients Infestation causes: • Bacteria • Fungus • Virus • Insect pests: Whiteflys, Mealybugs, Aphids, Spidermites, Nematodes) Fertilizer/Nutrients: • Nitrogen (N) • Phosphorus (P) • Potassium (K) 3 Ref: @Estación Experimental del Zaidín (CSIC), Spain Plant-Abiotic Interaction: • Drought (water deficit), • Excessive watering (water-logging/flooding), • Extreme temperatures (cold, frost and heat) • Salinity (sodicity) • Mineral (metal and metalloid) toxicity 4 Combined biotic and abiotic environmental stress factors: Healthy Stressed 4 Crop health via Reflectance profiling: • Vegetation stress identification and monitoring • Detection of crop responses to biotic and abiotic stressors Healthy Stressed Healthy Unhealthy (high biomass) (low biomass) 4 Ref: @Basics of Crop Sensing, ANR-1398 Reflectance profiling: Healthy vs. Unhealthy/Stressed Plants (chlorophyll content or plant health) Plants reflect Green light and absorb all other colors (Blue, Red etc.) 5 Processing and Analysis Steps: Raw HRS Data Data Calibration Performance Hypercube Preference Spectral Profile Bioassay LICOR (Reflectance) Nutrition Analysis Data Analysis/ Solution Root Analysis 3 Benchtop Hyperspectral Camera: Sensor Type: Push-broom line scanning Spectral bands: 240 Range: 380-1035nm Spectral resolution: 2.75 nm Hypercam Plants Hypercube 6 Ref: @Nansen Lab., UC Davis
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