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Genetic Relationships (RAPD-PCR) Between Geographically Separated Populations of the "Cosmopolitan" Interstitial Polychaete Hesionides gohari (Hesionidae) and the Evolutionary Origin of the Freshwater Species Hesionides riegerorum PDF

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Preview Genetic Relationships (RAPD-PCR) Between Geographically Separated Populations of the "Cosmopolitan" Interstitial Polychaete Hesionides gohari (Hesionidae) and the Evolutionary Origin of the Freshwater Species Hesionides riegerorum

Reference: Binl. Bull. 196: 216-226. (April 1999) Genetic (RAPD-PCR) Between Relationships Geographically Separated Populations of the "Cosmopolitan" Interstitial Polychaete Hesionides gohari (Hesionidae) and the Evolutionary Origin of the Freshwater Hesionides Species riegerorum HARTMUT SCHMIDT AND WILFRIED WESTHEIDE* Spezielle Zoologie, Biology/Chemistry, University ofOsnahriick. 49069 Osnabriick, Germany Abstract. In an analysis of the population genetics ofthe Introduction tiny meiofaunal polychaete Hesionides gohari. the RAPD- PCR method was applied to 49 specimens from 7 collecting The many species of Hesionides Friedrich, 1937, are sites far apart on three continents: French Atlantic coast, highly characteristic meiofaunal polychaetes with a great Mediterranean (Majorca, Giglio, Crete), Red Sea, Indian variety of morphological, reproductive, and behavioral ad- Ocean (Phuket), and U.S. Atlantic coast (Florida). In the aptations to the habitat of interstitial crevices in marine band patterns produced with 14 arbitrary decamer primers, surf-beaten sand beaches (Westheide, 1967, 1971, 1984). 496 genetic characters were detected. Genetic distances Several of the Hesionides species are known to be charac- between the H. gohari populations vary between 0.55 and terized by another pecularity of the marine interstitial 0.70. The data were evaluated by three cluster programs; in fauna a broad geographic distribution, comprising inter- the almost congruent phenograms, three clades were found tidal localities throughout the world (Westheide, 1971: Ster- rer. 1973; Giere, 1993). Within the genus, H. arenaria with high bootstrap values: ( 1 ) European Atlantic-Mediter- Friedrich, 1937, and H. gohari Hartmann-Schroder, 1960, ranean-Red Sea. (2) Indian Ocean, (3) Western Atlantic. In appear to have the most cosmopolitan distribution (West- all clusteranalyses. Hesionides riegerorum from aU.S. east heide, 1977); they occuron allcontinents except Antarctica. coast river system is shown as genetically nearest to the H. gohari wasdiscovered by AdolfRemane in littoral sands Florida specimens of H. gohari, making it most probable near Ghardaqa (today: Hurghada) in the Red Sea (Remane that this freshwater species of the genus originated from a and Schulz, 1964); it was described by Hartmann-Schroder Western Atlantic H. gohari population. The genetic dis- (1960). Westheide (1970, 1972a, 1972b) investigated the tances detected between the H. gohari specimens from the morphology, reproductive biology, and local distribution threecontinents are almost identical to those found between pattern of populations occurring in beaches of northern morphologically similarinterstitial polychaete species pairs. Tunisia (Mediterranean Sea). Thus, the degree ofgenetic consistency is considered not to This tiny species (max. length 1.5 mm) is typical ofwarm be high enough to corroborate the notion ofa cosmopolitan seas and has a range extending as far north as Arcachon, on distribution pattern, but rather suggests that the three clades the Atlantic coast of France (Westheide. 1972/73). New represent different species. localities for the species are still being recorded worldwide (Hartmann-Schroder, 1991; Westheide. 1992). Its most characteristic diagnostic features are considered to be its length, the proportions ofthe head appendages, the position Received 9 September 1998; accepted 29 January 1999. of penis papillae, the dentation of notopodial chaetae, and *To whom correspondence should he addressed. E-mail: westheide(S' especially the shape of the anal appendages. Among the mail.biologie.uni-osnabrueck.de other Hesionides species of comparable size, one inhabits 216 POPULATION GENETICS OF A MEIOFAUNAL POLYCHAETE 217 Figure 1. Records (opencirclesl and sampling sites (solidcircles)forHesionidesgohariand sampling site for H. riegemrum (triangle). fresh water, H. riegerorum Westheide, 1979: it can be uals in the seawatercolumn along shores has been observed distinguished from H. gohari by various morphological and demonstrated experimentally (Hagerman and Rieger, features. 1981; Palmer, 1988; Armonies. 1989). These observations The demonstrated almost cosmopolitan distribution of ledtothe proposal that the cosmopolitan distribution pattern these Hesionides species, together with that of many other is ascribable not togeologically ancient processesbut rather species of various meiofaunal taxa, gave rise to a still- to occasional contemporary events (Sterrer, 1973: Gerlach. controversial hypothesis of Sterrer (1973) (see also Rao, 1977) involving long-range dispersal by birds, on drifting 1972) based on the notion that speciation of these forms is material, or in ballast sand or tanks in ships. extraordinarily slow. It is postulated that they were already Lately, arguments for one or the other view have been present on an old supercontinent and were distributed over supported by genetic investigations. Todaro el al. (1996) their present vast range by the drifting apart of the conti- expressed the conviction, based on their restriction-frag- nental plates. This explanation could apply only if the ment length polymorphism analyses, that the proposed cos- genetic changes in the separated populations were relatively mopolitan species Xenotrichula intermedia (Gastrotricha), small overlong periods oftime, and mostly had noeffect on apparently present both in the Mediterranean Sea and along the phenotype. Such minimal genetic variability can be the coast of North America (Ruppert, 1977), consists of explained by assuming a complete constancy ofthe ecolog- more than one taxon. Similarly, with RAPD-PCR (random ical factors in the habitat ofthese animals, the sand beaches. applied polymorphic DNA-polymerase chain reaction) Sterrer's hypothesis appeared necessary because long- Soosten et til. (1998) found genetic differences between range, transoceanic dispersal ofthese meiofaunal organisms specimens of the polychaete Petitia amphophthalma from seemed inconceivable: they are not capable ofactive swim- Europe and North America, although these differences are ming and, with few exceptions, have no larval dispersal considerably smaller than those between certain morpho- stages. logically distinct species ofother taxa. In the present study However, it gradually became evident that, even without of Hesionides gohari collected from seven intertidal local- dispersal stages, many of these species do succeed in colo- ities on three continents (Europe, North America, Southeast nizing geologically young islands farfrom any coast (West- Asia; Fig. 1), the RAPD-PCR method was likewise chosen, heide. 1991): furthermore, dispersal of meiofaunal individ- to evaluate the results obtained for P. amphophthalma with 218 H. SCHMIDT AND W. WESTHEIDE Table I Collection informationforH. gohari and H. riegerorum Species POPULATION GENETICS OF A MEIOFALINAL POLYCHAETE 219 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Figure 2. RAPD fingerprinting ofHesionidesgohari specimens from Phuket (2. 31, Florida (8-11), Crete (12-151.Giglio(16-18).Arcachon(19-23).andH. riegerorumspecimensfromNorthCarolina(4-7); 1 and24; 100 bp marker. Primer OPB 6. Technologies, Inc., Berlin, Germany, or Pharmacia Biotech, and Sokal. 1973), and neighbor joining (Saitou and Nei, Uppsala, Sweden). The reproducibility of the data was 1987) including bootstrap proportions (Felsenstein. 1985). checked at regular intervals on different levels throughout all three parallel experiment lines. First, at the beginning of Results each series the optimal DNA concentration for PCR was determined for the individual animal, by testing the PCR For the seven sampled populations ofH. gohari and one with three DNA concentrations per animal (cci. 1 ng, 3 ng, population of H. riegerorum, with 14 different primers a and 10 ng per 25 jul reaction volume). In pilot experiments total of 496 different DNA fragments were detected, rang- with larger annelids, it became clear that the results were ing in length from 150 bp to 1900 bp (e.g., Fig. 2). reproducible with up to 25 ng/25 /u,l reaction volume. With The calculated degree of polymorphism is similarly high DNA concentrations as high as ca. 50 ng/25 /u.1 reaction in all the populations tested: for Arcachon, 96%; Giglio, volume, reproducibility was distinctly worse. A further in- 94%; Majorca, 95%; Crete, 94%; Hurghada, 89%; Phuket, crease in DNA concentration (to over 100 ng/25 ju.1 reaction 89%; Florida, 97%; and for H. riegerorum of North Caro- volume) brought the PCR to a complete halt, so that no lina, 86%, the lowest value. For none of the European specific DNA fragments could be detected. Reproducibility populations, nor for the population at the Red Sea, could DNA was tested furtherthroughouteach test series by running the diagnostic fragments be detected. However, if the same experimenttwice in parallel reactions. In addition, for European and Egyptian animals are considered as a single each series one reaction was carried out with a "blind" group, its members are found to exhibit 33 common poly- sample lacking DNA, to check the possibility of contami- morphic characters that are absent from the individuals nation with foreign DNA. We also looked fordifferences in living in Thailand and Florida. With few exceptions, char- the amplification patterns that might derive from the use of acters present in the animals from Thailand are the same as different thermocyclers (Biischer et al., 1993; He et al.. those found in the European and Egyptian individuals. DNA 1994) or preservation methods (drying or deep-freezing), Three diagnostic and five polymorphic fragments but found none. were observed in the Phuket population only. Only one The detected amplification product patterns were exam- characteristic polymorphic character was detectable in the ined visually for monomorphic and polymorphic markers animals from Florida, and none of the primers revealed (Hadrys et al.. 1992). The degrees of polymorphism are diagnostic DNA fragments. A surprising result was ob- given in percentages. The banding patterns were then trans- tained by comparing the population of H. riegerorum with lated into a 0/1-matrix (0 for absence, 1 for presence of a those ofH. gohari. Although this freshwater species can be specific DNA marker) and fed into the cluster analysis distinguished from the H. gohari populations by 12 diag- program TREECON 1.2 (Peer and Wachter, 1994), which nostic DNA fragments and 9 polymorphic characters, all alsotransformed thedataintodistance values in percentages other DNA bands are common to the two groups. There is (Nei and Li, 1979). Cluster analyses were carried out by an especially close match with the animals of the Florida UPGMA (unweighted pair-group method using arithmetic population: of the 191 shared characters, 39 polymorphic averages; Sneath and Sokal, 1973), single linkage (Sneath DNA fragments are present only in these individuals. The 220 H. SCHMIDT AND W. WESTHEIDE Table III Comparison ofgeneticdistancesgeneratedfrom RAPDdata (afterNeiandLi, 19791 within andbetiveen the seven Hesionides goharipopulationsand thf solepopulation ofHesionides riegerorum Locality POPULATION GENETICS OF A MEIOFAUNAL POLYCHAETE 221 0.1 98 222 H. SCHMIDT AND W. WESTHEIDE 0.1 94_ 94 \ POPULATION GENETICS OF A MEIOFAUNAL POLYCHAETE 223 0.1 224 H. SCHMIDT AND W. WESTHEIDE (Bochert, 1997), whereas H. gohari is thought to undergo although considerably less than that between the latter and direct development (Westheide, 1970). Along-shore migra- the gohari individuals from Thailand or Europe. But tion is presumably a general feature ofthe latter species, but whereas the freshwater species is also clearly distinguish- the rate ofgeographic expansion achieved by this means is able morphologically (e.g.. bythe different notopodial chae- unknown. tae. penis papillae, and anal lobes; Westheide, 1979), no H. gohari is considered to be one of the many cosmo- such diagnostic characters have yet been identified for the politan interstitial polychaete species (Westheide, 1971; populations classified as H. gohari. The small geographic Sterrer. 1973: Westheide and Rao. 1977; Riser. 1981; Giere. distance between the new freshwater species and its marine 1993; Soosten et at., 1998). As yet no morphological dis- population of origin has evidently produced morphological tinctions have been found among individuals from the most character shifts, some of which may act as isolating mech- diverse ofthe earth's marine regions (see Fig. 1), so that the anisms for instance, the differently positioned and shaped species must temporarily be regarded as distributed world- penis papillae on the prostomium (see also Westheide, wide on the coasts of warm seas (Westheide. 1977; Hart- 1984, fig. 8). In contrast, the large geographic distance mann-Schroder, 1991) provided that a morphological spe- separating the European/Egyptian, Thai, and North Ameri- cies concept is applied. In the present genetic study, all the can individuals from one another is correlated with genetic cluster analyses employed show clear differences between distinctions but not with any morphological ones that can the animals in the regions (1 ) European Atlantic/Mediter- readily be discerned, perhaps because ofthe stability ofthis ranean/Red Sea. (2) Indian Ocean, and (3) Western Atlantic marine habitat. (North America). Whether the observed genetic distances To continue to regard all these populations as belonging reflect species differences that is, reproductive barriers to a single species. H. gohari, is problematic. They would cannot be decided on the basis of current observations. In then constitute something like a paraphylum (Lorenzen, another cosmopolitan morphospecies, Petitia amphoph- 1976). with one population (Florida) more closely related to rlmlina Slewing. 1956 (Syllidae). the distances found by the distinct sibling species (H. riegerorum) than to the other RAPD analyses between North American and European populations. As soon as possible, therefore, at least the animals correspond to the values found here: 0.60-0.66 North American "//. gohari" should be given the status ofa (Soosten et ai, 1998). However, similar distance values separate species. However, we prefer not to undertake its (after Nei and Li, 1979) of geographically separated but description until the animals on the coast of the United morphologically differentiated pairs of interstitial poly- States have been further examined in a specific search for chaete species have been revealed by RAPD analyses as morphological diagnostic characters, so that the practical well: Nerilla antennata Schmidt, 1848-/V. mediterranea taxonomic work will not be hampered by a species analysis Schlieper, 1925 (Nerillidae): 0.74-0.77 (Schmidt and based exclusively on genetic tests and on the geographic Westheide. 1998): Microphthalmus carolinensis West- situation. heide and Rieger, 1987-A/. nahuntensis Westheide and Regarding Sterrer's (1973) hypothesis, it is irrelevant Rieger, 1987: 0.77 (unpubl. data). Schirmacher et al. whether these genetically derived clades represent distinct (1998) found a distance value of only 0.17 between two species or merely populations ofa single, and hence indeed enchytraeid sibling species. Thus the notion ofH. gohari cosmopolitan, species. Testing this question, however, de- representing acosmopolitan species will have to be aban- mands a larger number of specimens from different sites doned. separated by oceans and sequencing techniques. It is remarkable in this connection that the Florida pop- ulation, identified as H. gohari, in all analyses formed a sibling cluster with the freshwater species H. ricgeronmi Acknowledgments Westheide, 1979. These animals are the nearest neighbors geographically to the latter species, with recorded finds on Special thanks for the provision ofworking facilities are the East Coast of the United States in Florida (Westheide. due to Mr. Somsak Chullasorn and Dr. Anuwat Natee- 1995) and North Carolina; that is, they live only about 100 wathana (Phuket Marine Biological Center), Dr. Mary Rice km away from the habitat of the freshwater species, which (Smithsonian Institution at Harbor Branch, Ft. Pierce. Flor- so farhas been found only at a site in the sandy bank ofthe ida), Dr. Charles H. Petersen and Hal Summerson (Marine Chowan River in North Carolina. When H. riegerorum was Science Institute. Morehead City. North Carolina), Dr. first described, mention was made ofits close morphologi- Claude Cazaux (Laboratoire Marine. Institut Universitaire cal resemblance to H. gohari (Westheide. 1979). There is de Biologic marine de Bordeaux. Arcachon), and Mrs. Mar- much evidence that the freshwater form separated from the tina Ubel (Institut fur Marine Biologic. Giglio). Among the neighboring marine Hesionides population on the seacoast. various people who helped in the collection of the speci- The genetic distance between H. riegerorum and the marine mens, we especially thankCornelia von Soosten, MonikaC. Hesionides individuals on the Florida coast is appreciable. Miiller, and Alice Westheide. We gratefully acknowledge POPULATION GENETICS OF A MF.IOFAUNAL POLYCHAETE 225 preparation and typing of the manuscript hy Mrs. Anna Riser, N. W. 1981. The aberrant polychaete Slygocniiitella from some Stein and Mrs. Andrea Noel. American beaches. Wasnmnn J. Biol. 38: 10-17. Ruppert, K. E. 1977. Zoogeography and speciation in marine Gastro- tncha. in TheMeiofaunaSpeciesin TimeandSpace. W. Sterrerand P. Ax. eds. Workshop Symposium, Bermuda. 1975. Mikrofauna Meeres- Literature Cited hodcn 61: 231-251. \tmimics. W. 1989. Meiot'aunal emergence from inlertidal sedimenl Saitou, N., and M. Nei. 1987. The neighbourjoining method. A new measured in the Held: significant contribution to nocturnal planktonic method forreconstructing phylogenetictrees. Mol. Biol. Evol. 4: 406- hioiiKiss m shallow waters. Helgol. Meeresunters. 43: 29-43. 425. Baslrop, R., M. Riihner, C. Sturnihauer, and K. Jiirs.s. 1997. Where Schierwater, B. 1995. Arbitrarily amplified DNA in systematic* and didMarenzelleria spp. (Polychaeta: Spionidae) in Europe come from? phylogenetics. Electrophoresis 16: 1643-1647. Ai/uat. Ecol. 31: I 14-136. Schirmacher, A., H. Schmidt, and W. Westheide. 1998. RAPD-PCR Bochert, R. 1997. Maren:elleria riridis (Polychaeta: Spionidae): a re- investigations on sibling species ofterrestrial Enchyirueiis (Annelida: Mew ofits reproduction. Ai/uat. Ecol. 31: 163-175. Oligochaeta). Binchem. Syst. Ecol. 26: 27-36. Biischer, N., E. Zyprian, and R. Blaich. 1993. Identification of Schmidt, H., and VV. Westheide. 1998. RAPD-PCR experiments con- grapewme cultivars by DNA analyses: pitfalls of random amplified firm the distinction between three morphologically similar species of polymorphicDNAtechniquesusing lOmerprimers. Vitis32: 187-188. Nerilla (Polychaeta: Nerillidae). Zool. An:. 26: 277-285. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach Sneath,P. H. A., and R. R. Sokal. 1973. Numerical Taxonomy. W. H. using the bootstrap. Evolution 39: 7778-7783. Freeman, San Francisco. Gerlach, S. A. 1977. Means ofmeiofauna dispersal. In The Mcinfmuw Soosten, C. von, H. Schmidt, and W. Westheide. 1998. Genetic vari- Species in Time and Spare. W. Sterrer and P. Ax. eds. Workshop ability and relationships among geographically widely separated pop- Symposium. Bermuda. 1975. Mikrofauna Meeresboden 61: 89-103. ulationsofPeliliaamphophthalma(Polychaeta: Syllidae):Resultsfrom Giere, O. 1993. \leiobenihology. The Microscopic Fauna in Aauaiu RAPD-PCR investigations. Mar. Biol. 131: 659-669. Sediments. Springer. Berlin. Sterrer, W. 1973. Plate tectonics as a mechanism for dispersal and Hadrys, H., M. Balick, and B. Schierwater. 1992. Application of speciation in interstitial sand fauna. Neth. J. Sea Res. 1: 200-222. random amplified polymorphic DNA (RAPD) in molecular ecology. Todaro,M. A.,J. W. Fleeger,Y. P. Hu,A. W. Hrincevich,and D. W. Mol. Ecol. 1: 55-63. Foltz. 1996. Are meiofaunal species cosmopolitan'.' Morphological Hagerman, G. M., and R. M. Rieger. 1981. Dispersal of benthic and molecular analysis of Xenolrichula intermedia (Gastrotricha: meiofaunabywaveandcurrentactioninBogueSound,NorthCarolina. Chaetonotida). Mar. Biol. 125: 735-742. USA. Puhhl. Srn. Zoo/. Napoli (/. Mar. Ecol.) 2: 245-270. Westheide, W. 1967. Monographic derGattungen Hesionides Friedrich Hartmann-Schroder. G. 1960. Polychaeten aus dem Roten Meer undMicrophthalmusMecznikow (Polychaeta). Ein Beitrag zuOrgani- KielerMeeresfurscli. 16: 69-125. sation und Biologic psammobionter Polychaeten. Z. Morph. Tiere 61: Hartmann-Schriider, G. 1991. Die Polychaeten der subtropisch-tropi- 1-159. schen bis tropischen Ostkiiste Australiens zwischen Maclean (New Westheide, W. 1970. Zur Organisation, Biologie und Okologie des South Wales) und Gladstone (Queensland) sowie von Heron Island interstitellen Polychaeten Hesionidesgohari Hartmann-Schroder (He- (GroBesBarrierRiff).In:G. Hartmann-SchroderandG. Hartmann.Zur sionidae). Mikrofauna Meeresboden 3: 1-37. Kenntnis des Eulitorals der australischen Kiisten unter besonderer Westheide, W. 1971. Interstitial Polychaeta (excluding Annelida). Berticksichtigung der Polychaeten und Ostracoden. Teil 16. Min. Smithson. Contrib. Zool. 76: 57-70. Hambg. ZoolMus. lust. 88: 17-71. Westheide, W. 1972/73. Nouvelles recoltes d'anne'lides interstitielles He, Q.. M. K. Viljanen, and .1. Mertsola. 1994. Effects ofthermocy- danslesplagessableusesduBassind'Arcachon. VieMilieuSerieA 23: clers andprimerson the reproducibility ofbanding patterns in random 365-370. amplified polymorphic DNA analysis. Mol. Cell. Probes 8: 155-160. Westheide, W. 1972a. Raumliche und zeitliche Differenzierungen im Kocher, T. D., W. K. Thomas, A. Meyer, S. V. Edwards, S. Paabo, Verteilungsmuster der marinen Interstitialfauna. Verh. Dtsch. Zool. F.X.Villablanca,and A. C. Wilson. 1989. Dynamicsofmitochon- Ges. 1971: 23-32. dria! DNA evolution in animals amplification and sequencing with Westheide, W. 1972b. La faune des Polychetes et des Archiannelides conserved primers. Proc. Nail. Acini. Sci. USA 86: 6196-6200. dans les plages sableuse a ressac de la cote mediterraneenne de la Lorenzen,S. 1976. ZurTheoriederphylogenetischenSystematik. Verh. Tunisie. Bull. lust. Natl. Sci. Tech. Oceanogr. Peclie. Salammbo 2: Dtsch. Zool. Ges. 1976: 22X 449-46S. Nei, M.,and W. H. Li. 1979. Mathematical model forstudying genetic- Westheide, W. 1977. The geographic distribution of interstitial variation in terms of restriction endonucleases. Genetics 76: 5269- polychaetes. In TheMeiofaunaSpecies in TimeandSpace. W. Sterrer 5273. and P. Ax. eds. Workshop Symposium, Bermuda. 1975. Mikrofauna Palmer, M. A. 1988. Dispersal of marine meiofauna: a review and Meeresboden 61: 287-302. conceptual model explaining passive transport and active emergence Westheide, W. 1979. Hexionides riegcrorum n. sp.. a new interstitial with implications forrecruitment. Mar. Ecol. Prog. Ser. 48: 81-91. freshwater polychaete form the United States. Int. Rev. Gesaintcn Peer,Y.vande,and R.deWachter. 1994. TREECONforWindows: a Hydrohiol. 64: 273-280. software package for the construction and drawing of evolutionary Westheide, W. 1984. The concept of reproduction in polychaetes with trees for the Microsoft Windows environment. Compiit. Appl. Biosci. small body size: adaptations in interstitial species. In Polvchaete Re- 10: 569-570. production, A. Fischer and H.-D. Pfannenstiel, eds. Fortsch. Zool. For,F.D. 1978. Lessepsian Migration. TheInfluxofRedSeaBiota into 29:265-287. theMediterraneanhy WayoftheSue:.Canal. Springer. Berlin 228pp. Westheide, W. 1990. Polychaetes: Interstitial Families. In Synopsis of Rao,G.C. 1972. Onthegeographicaldistributionofinterstitial faunaof the British Fauna {New series) No. 44. D. M. Kermack and R. S. K. marine sand. Proc. Indian Natl. Sci. Acad. 38B: 164-178. Barnes, eds. Universal Book Services/W. Backhuys. Oegstgeest. The Remane, A., and E. Schulz. 1964. Strand/onen des Roten Meeres und Netherlands. ihre Tierwelt. KielerMeeresforsch. 20: 5-17. Westheide, W. 1991. The meiofauna of the Galapagos. A review. Pp.

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