CATALOGUING AND MANAGEMENT OF MAJOR DISEASES OF MONOPODIAL ORCHIDS By MEERA T.M. (2010-11-122) Department of Plant Pathology COLLEGE OF HORTICULTURE VELLANIKKARA, THRISSUR - 680 656 KERALA, INDIA 2012 CATALOGUING AND MANAGEMENT OF MAJOR DISEASES OF MONOPODIAL ORCHIDS By MEERA T.M. (2010-11-122) THESIS Submitted in partial fulfillment of the requirement for the degree of Master of Science in Agriculture Faculty of Agriculture Kerala Agricultural University Department of Plant Pathology COLLEGE OF HORTICULTURE VELLANIKKARA, THRISSUR - 680656 KERALA, INDIA 2012 DECLARATION I, hereby declare that the thesis entitled “Cataloguing and management of major diseases of monopodial orchids.” is a bonafide record of research work done by me during the course of research and that it has not been previously formed the basis for the award to me of any degree, diploma, fellowship or other similar title, of any other University or Society. Vellanikkara 17-08-12 Meera T.M. Dr.Vimi Louis Date: 17-08-12 Associate Professor Department of Plant Pathology College of Horticulture CERTIFICATE Certified that this thesis entitled “Cataloguing and management of major diseases of monopodial orchids.” is a bonafide record of research work done independently by Ms. Meera T.M. (2010-11-122) under my guidance and supervision and that it has not formed the basis for the award of any degree, diploma, fellowship or associateship to her. Vellanikkara Dr. Vimi Louis Chairperson Advisory Committee CERTIFICATE We, the undersigned members of the advisory committee of Ms.Meera T.M. (2010-11- 122) a candidate for the degree of Master of Science in Agriculture with major field in Plant Pathology agree that this thesis entitled “Cataloguing and management of major diseases of monopodial orchids” may be submitted by Ms. Meera T.M., in partial fulfillment of the requirement for the degree. Dr.Vimi Louis Associate Professor Dept. of Plant Pathology College of Horticulture Vellanikkara (Chairperson) Dr. T.J. Rehumath Niza Dr. S. Beena Professor & Head Professor Dept. of Plant Pathology Dept. of Plant Pathology College of Horticulture College of Horticulture Vellanikkara, Thrissur Vellanikkara, Thrissur (Member) (Member) Dr. C.K. Geetha Professor Dept. of Pomology & Floriculture College of Horticulture Vellanikkara, Thrissur (Member) ACKNOWLEDGEMENT First and foremost I bow my head before the Almighty God who enabled me to successfully complete the thesis work in time. With immense pleasure I avail this opportunity to express my deep sense of reverence, gratitude and indebtedness to my major advisor Dr.Vimi Louis, Associate Professor, Department Plant Pathology and Chairperson of my Advisory Committee for her valuable guidance, constant support, unfailing patience, friendly approach and timely help at various stages of my work and thesis preparation. I deeply express my whole hearted thanks to Dr. T.J. Rehumath Niza, Professor and Head, Department of Plant Pathology, College of Horticulture, Vellanikkara and member of my advisory committee for her wholehearted cooperation, valuable suggestions during various stages of the study and critical scrutiny of the manuscript. I would like to express my extreme indebtedness and obligation to Dr. S. Beena, Professor, Department of Plant Pathology and member of my advisory committee for her meticulous help, unwavering encouragement, forbearance, well timed support and critical scrutiny of the manuscript which has helped a lot for the improvement and preparation of the thesis. I sincerely thank Dr. C.K. Geetha, Professor, Department of Pomology and Floriculture, College of Horticulture, Vellanikkara and member of my Advisory Committee for valuable suggestions, well timed mental support and help rendered. I am deeply obliged to Dr. Sally K. Mathew, Dr. T. Sheela Paul and Dr.Sainamol Kurien whose constant help, love and support have helped me a lot to complete this venture successfully. I wish to express my gratitude to Sri. S. Krishnan, Assistant Professor, Department of Agricultural Statistics for his guidance throughout the statistical analysis of data. I truthfully thank Dr. P.K. Rajeevan, Professor and Head, Department of Pomology and Floriculture for his support during the research work. I am deeply obliged to Dr. Haseena Bhaskar, Associate Professor, Department Of Agricultural Entomology for her help in the preparation of photographs. I thank my dear friends Remya and Yunus and juniors Roshna and Priyadarshini and all my M.Sc. Classmates Binisha, Chandu, Jimcy, Fahi, Priya, Roshin, Smitha, Adheena, Athul, Najeeb, Sayooj, Varun and Negi for their moral support and encouragement. I take this opportunity to thank my seniors Mrs.Hajara, Ms.Sreeja, Ms.Divya, Mrs. Jyothi, Mrs.Kaveramma, Mrs.Deepa and Mrs. Gleena for their support and encouragement. I extend my loving gratitude to Ms.Jisha, Ms.Sindu, Mrs.Jisha, Mrs.Mini, Mrs.Shanta, Mrs.Shobha , Ms.Glindhya and Ms.Aparna for their timely help whenever I was in need of their moral support and encouragement. I thank Mr. Aravind, Computer Club, College of Horticulture, for his valuable help in computer work. I am deeply indebted to my Parents and brother without whose moral support, blessings and affection this would not have been a success. It would be impossible to list out all those who have helped me in one way or another in the successful completion of this work. I once again express my heartful thanks to all those who helped me in completing this venture in time. Meera T.M CONTENTS Chapter Title Page No. 1 INTRODUCTION 1-2 2 REVIEW OF LITERATURE 3-19 3 MATERIALS AND METHODS 20-28 4 RESULTS 29-70 5 DISCUSSION 71-86 6 SUMMARY 87-90 REFERENCES i-ix APPENDIX ABSTRACT LIST OF TABLES Table Title Page No. No. 1 Score chart for disease severity 25 2 Treatments used for in vitro evaluation against fungal pathogens 27 3 Treatments used for in vitro evaluation against bacterial pathogens 28 4 Diseases of monopodial orchids observed during survey 29 5 Fungal and bacterial pathogens isolated from diseased specimens 31 6 Identification of fungal pathogens 44 7 Per cent incidence of diseases of Phalaenopsis 47 8 Per cent incidence and severity of leaf spot diseases of Basket Vanda, 50 Mokara and Arachnis 9 Temperature and relative humidity in rain shelter from May’11 to April’12 51 10 Temperature and relative humidity in poly house from July’11 to June’12 51 11 Weather data at monthly intervals from July 2011 to June 2012 52 12 In vitro evaluation of fungicides and P. flourescens against Fusarium 55 oxysporum from Phalaenopsis 13 In vitro evaluation of fungicides and P. flourescens against Sclerotium 56 rolfsii from Phalaenopsis 14 In vitro evaluation of fungicides and P. flourescens against Phoma exigua 57 from Phalaenopsis 15 In vitro evaluation of fungicides and P. flourescens against Colletotrichum 59 gloeosporioides from Phalaenopsis 16 In vitro evaluation of fungicides and P. flourescens against Phytophthora 60 cactorum from Basket Vanda 17 In vitro evaluation of fungicides and P. flourescens against Sclerotium 61 rolfsii from Basbket Vanda LIST OF TABLES Table Title Page No. No. 18 In vitro evaluation of fungicides and P. flourescens against 63 Botryodiplodia theobromae from Basket Vanda 19 In vitro evaluation of fungicides and P. flourescens against 64 Colletotrichum gloeosporioides from Mokara 20 In vitro evaluation of fungicides and P. flourescens against Alternaria 65 alternata from Mokara 21 In vitro evaluation of fungicides and P. flourescens against 67 Colletotrichum gloeosporioides from Arachnis 22 In vitro evaluation of fungicides and P. flourescens against Fusarium 68 oxysporum from Arachnis 23 In vitro evaluation of antibiotic, cowdung and P. flourescens against 69 Erwinia chrysanthemi from Phalaenopsis 24 In vitro evaluation of antibiotic, cowdung and P.flourescens against 69 Burkholderia gladioli from Basket Vanda
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