Biotechnology for Sustainable Agriculture This pageintentionallyleftblank Biotechnology for Sustainable Agriculture Emerging Approaches and Strategies Edited by Ram Lakhan Singh Sukanta Mondal WoodheadPublishingisanimprintofElsevier TheOfficers’MessBusinessCentre,RoystonRoad,Duxford,CB224QH,UnitedKingdom 50HampshireStreet,5thFloor,Cambridge,MA02139,UnitedStates TheBoulevard,LangfordLane,Kidlington,OX51GB,UnitedKingdom Copyrightr2018ElsevierInc.Allrightsreserved. Nopartofthispublicationmaybereproducedortransmittedinanyformorbyanymeans,electronicor mechanical,includingphotocopying,recording,oranyinformationstorageandretrievalsystem,without permissioninwritingfromthepublisher.Detailsonhowtoseekpermission,furtherinformationaboutthe Publisher’spermissionspoliciesandourarrangementswithorganizationssuchastheCopyrightClearance CenterandtheCopyrightLicensingAgency,canbefoundatourwebsite:www.elsevier.com/permissions. ThisbookandtheindividualcontributionscontainedinitareprotectedundercopyrightbythePublisher (otherthanasmaybenotedherein). Notices Knowledgeandbestpracticeinthisfieldareconstantlychanging.Asnewresearchandexperience broadenourunderstanding,changesinresearchmethods,professionalpractices,ormedicaltreatment maybecomenecessary. Practitionersandresearchersmustalwaysrelyontheirownexperienceandknowledgeinevaluatingand usinganyinformation,methods,compounds,orexperimentsdescribedherein.Inusingsuchinformation ormethodstheyshouldbemindfuloftheirownsafetyandthesafetyofothers,includingpartiesfor whomtheyhaveaprofessionalresponsibility. Tothefullestextentofthelaw,neitherthePublishernortheauthors,contributors,oreditors,assumeany liabilityforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliability,negligence orotherwise,orfromanyuseoroperationofanymethods,products,instructions,orideascontainedin thematerialherein. BritishLibraryCataloguing-in-PublicationData AcataloguerecordforthisbookisavailablefromtheBritishLibrary LibraryofCongressCataloging-in-PublicationData AcatalogrecordforthisbookisavailablefromtheLibraryofCongress ISBN:978-0-12-812160-3(print) ISBN:978-0-12-812238-9(online) ForinformationonallWoodheadPublishingpublications visitourwebsiteathttps://www.elsevier.com/books-and-journals Publisher:AndreWolff AcquisitionEditor:NancyMaragioglio EditorialProjectManager:KarenMiller ProductionProjectManager:SuryaNarayananJayachandran CoverDesigner:GregHarris TypesetbyMPSLimited,Chennai,India Dedication Dedicated to the students and researchers who refined our knowledge of Biotechnology by their intelligent questions, queries, and discussions over the years. This page intentionallyleftblank Contents ListofContributors xv Preface xvii Acknowledgments xxi 1. Introduction 1 RamLakhanSinghandSukantaMondal Biotechnology 2 SustainableAgriculture 3 ApplicationofBiotechnologyforSustainableAgriculture 4 GeneticallyModifiedFoods 5 BiotechnologyforSustainableLivestockProduction 6 BiotechnologiesforGutMicroorganism 10 UseofMicrobesforSustainableAgriculture 10 ImpactofClimateChangeonAgricultureandLivestockProduction 11 NanotechnologyforSustainableAgriculture 14 BiosafetyAspects 15 GeneticEngineeringandPublicPerception 15 References 16 2. Biotechnological Tools to Enhance Sustainable Production 19 RajatP.Singh,PradeepK.Singh,RasnaGuptaandRamLakhanSingh Introduction 19 NeedforBiotechnologicalToolsforSustainableAgricultural Production 20 BiotechnologicalToolsforCropImprovement 21 PlantBreedingandMarker-AssistedSelection 21 PlantTissueCultureandMicropropagation 23 GeneticEngineeringandGeneticallyModifiedCrops 24 MolecularPharming 26 Biofertilizers 28 Biopesticides 29 TypesofBiopesticides 29 Bacteria 30 Fungi 31 Baculovirus 31 vii viii Contents Plant-DerivedInsecticides 31 Neem 32 BiochemicalPesticides 33 BiotechnologicalToolsforLivestockImprovement 33 Marker-AssistedSelection 33 ReproductiveTechnologies 36 GeneticEngineeringandGMAnimals 41 MolecularDiagnosticToolsandAnimalHealth 46 VaccineTechnology 48 BiotechnologicalToolsforIndustrialApplication 53 ProteinEngineering(PE) 53 MetabolicEngineering(ME) 59 Conclusion 62 References 63 3. Sustainable Agriculture and Food Security 67 SharanaiahUmesha,HonnayakanahalliM.G.Manukumar andBhadveluChandrasekhar Introduction 67 TransferofTechnologies 71 InformationSupport 71 OrganicFarmingforSustainableAgriculture 73 BenefitsofSoilOrganicMatter 74 FoodSecurityandClimateChange 75 FourDimensionsofFoodSecurity 76 TheDurationofFoodInsecurity 76 Foodshed 82 TheFootprintofFood:The“Foodprint” 83 MetropolitanAgriFoodSystem 83 TheNeedtoImproveFoodSafetyandtoImplement QualityAssurance 85 The“Preharvest”FoodSafetyandQualityApproach 86 ImprovingNutritionOutcomeRequiresMoreThanFood 87 TheMostVulnerabilityFromanUnderNutritionPerspective 87 TheIntractable,EquityDebateonSustainableDiets 87 ClimateChangeAdaptionandMitigation 88 FutureofFoodandFarming:ChallengesandChoicesfor GlobalSustainability 89 Conclusion 91 References 91 4. Plant Biotechnology and Crop Improvement 93 PratikSatyaandDebabrataSarkar Introduction 93 BoostingSustainableCropProductionThrough BiotechnologicalApproaches 95 Contents ix EnhancingProductivityThroughGeneticallyModifiedCrops 96 MolecularPlantBreedingandGenomics-AssistedCrop Improvement 106 BiotechnologyforImprovingNutritionalQuality: TransgenicPlantsforBetterHumanHealth 115 GoldenRice 115 BiofortificationofIroninRice 116 QualityProteinMaize 117 ModificationofEdibleOilQuality 117 GenomeEditingforCropImprovement 118 ZincFingerNucleasesasFirstMolecularScissors 119 TALEN:MoreEfficientMolecularScissor 119 CRISPR/Cas9:TheMostEfficientScissor 119 MolecularPharmingforMetaboliteProductioninPlants 121 Therapeutic,Nutritional,andOtherIndustrialProteinProduction inPlant 121 SecondaryMetaboliteProductionbySingleCellCulture 123 FutureOutlook 124 References 125 FurtherReading 140 5. Transgenic Animal Production 141 Louis-MarieHoudebine Introduction 141 TechniquesforAnimalGeneticModifications 144 MechanismsofRandomGeneIntegration 144 MechanismsofTargetedGeneIntegration 145 MethodsforGeneTransfer 147 DNATransferIntoEmbryos 148 DNATransferIntoIntermediateCells 152 GeneConstruction 156 NucleotidicCompositionoftheVectors 159 AdditionofInsulators 159 OptimizationoftheTranscribedRegion 160 CoexpressionofTwoCistronsFromtheSameVector 162 GeneInactivation 162 ControlofTransgenesbyExogenousInducers 164 GeneDeletion 164 UseofTransgenicAnimals 165 BasicandMedicalResearch 165 AdaptationofPigOrgansforTransplantationtoHumans 166 ProductionofPharmaceuticalProteinsbyTransgenicAnimals 167 ImprovementofAnimalProduction 168 AcceptabilityofTransgenicAnimals 174 Perspectives 175 References 176
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