Alterations induced by gravity changes in proliferating culture cells of Arabidopsis thaliana Khaled

301 Pages·2014·17.22 MB·English

UNIVERSIDAD COMPLUTENSE DE MADRID
FACULTAD DE CIENCIAS BIOLÓGICAS
DEPARTAMENTO DE GENÉTICA

TESIS DOCTORAL

Alteraciones inducidas por cambios gravitatorios en células proliferantes en
cultivo de Arabidopsis thaliana

MEMORIA PARA OPTAR AL GRADO DE DOCTOR

PRESENTADA POR

Khaled Youssef Kamal Moustafa

Directores

Francisco Javier Medina Díaz
Raúl Herranz Barranco

Madrid, 2014

©Khaled Youseef Kamal Moustafa, 2014
UNIVERSIDAD COMPLUTENSE DE MADRID
FACULTAD DE BIOLOGÍA
DEPARTAMENTO DE GENÉTICA

Alteraciones inducidas por cambios
gravitatorios en células proliferantes en
cultivo de Arabidopsis thaliana

Khaled Youssef Kamal Moustafa

Licenciado en Ingeniería Agronómica

Madrid, 2014

Departamento de Biología Medioambiental
Centro de Investigaciones Biológicas
Consejo Superior de Investigaciones Científicas

UNIVERSIDAD COMPLUTENSE DE MADRID
FACULTAD DE BIOLOGÍA
DEPARTAMENTO DE GENÉTICA

Alterations induced by gravity changes in
proliferating culture cells of Arabidopsis
thaliana

Khaled Youssef Kamal Moustafa

Licenciado en Ingeniería Agronómica

Madrid, 2014

Departamento de Biología Medioambiental
Centro de Investigaciones Biológicas
Consejo Superior de Investigaciones Científicas

UNIVERSIDAD COMPLUTENSE DE MADRID
FACULTAD DE BIOLOGÍA
DEPARTAMENTO DE GENÉTICA

Alteraciones inducidas por cambios
gravitatorios en células proliferantes en
cultivo de Arabidopsis thaliana

(ALTERATIONS INDUCED BY GRAVITY CHANGES IN PROLIFERATING
CULTURE CELLS OF ARABIDOPSIS THALIANA)

Khaled Youssef Kamal Moustafa

Licenciado en Ingeniería Agronómica

Madrid, 2014

Bajo la dirección de los doctores: Tutor de la Facultad de
Francisco Javier Medina Díaz Ciencias Biológicas (UCM):
Raúl Herranz Barranco Francisco Javier Gallego Rodríguez

Vº Bº Directores de tesis doctoral: Vº Bº Tutor de doctorado:

Dr. Francisco Javier Medina Díaz Dr. Francisco Javier Gallego Rodríguez

Dr. Raúl Herranz Barranco

My Dear Mother

Mom, who behind and the secret of
my happiness
I Love You…..

Doctoral Thesis Acknowledgements

ACKNOWLEDGEMENTS

It gives me great pleasure in expressing my gratitude to all those people who have supported me
and their contributions in making this thesis possible. First and foremost, I must acknowledge and
thank The Almighty Allah for blessing, protecting and guiding me throughout this period. I could
never have accomplished this without the faith I have in Allah.

I express my profound sense of reverence to my supervisor and head of our laboratory Dr. Medina
for his constant guidance, support, motivation and untiring help during my predoctoral stay at CIB.
His in-depth knowledge on a broad spectrum of different microscope techniques and cellular
biology topics has been extremely beneficial for me. He has given me enough freedom to explore on
my own and at the same time the guidance to recover when my steps faltered. I will always
remember his calm and relaxed nature, and the way he asks “YES! How can I help you? “In English
instead of Spanish”, whenever I enter his office. I am thankful to the Allah for giving me a mentor
like him.
Furthermore, my deepest gratitude is to my co-supervisor, Dr. Raúl Herranz. Raúl taught me how to
question thoughts and express ideas that helped me sort out the technical details specially, genomic
and proteomic analysis. His patience and support helped me overcome many crisis situations and
finish this dissertation. I am also thankful to him for encouraging the use of correct grammar and
consistent notation in my writings and for carefully reading and commenting on countless revisions
of this manuscript.
I also would like to acknowledge my current and previous colleagues in the CIB lab; Miguel (the
person who help me most in my first steps in Spain, thank you so much Miguel), Aranzazu, the
newest student at our lab, and Dr. Manzano. Moreover, I will always be grateful to Mercedes for all
her technical help and lovely social lab interactions.
I would like to thanks all the member of the CIB services and departments who participated in the
works conducting to this thesis. Special thanks to Dr. Pedro Lastres for his help, discussion, and
work inspiration about flow cytometry analyses. Thanks a lot Pedro, without you almost the whole
thesis project will not been finished.
Khaled Youssef Kamal 2014 1